Immunological consequences of DTA-mediated deletion of CD134+CD4+ T cells. (a) Size of inguinal (iLN), axillary (aLN), brachial (bLN), cervical (cLN), mesenteric (mLN) lymph nodes and spleen (SP) from Tnfrsf4Cre/+ R26Dta/+ (DTA) and littermate control Tnfrsf4Cre/+ R26+/+ (WT) mice. (b) Serum levels (mean ± SEM, n = 5-7) of MCP-1, IL-12 (p40), IFN-γ, MIP-1α, IP-10 (CXCL10), IL-1β and MIG (CXCL9) in the same mice. (c) Total numbers of B cells, T cells and macrophages (Mphi). P = 0.02 and P = 0.03 for total cells and B cells, respectively. (d) CD4:CD8 ratio. (e) Total numbers (mean, n = 9-12) of naïve, memory and regulatory (reg) CD4+ T cells and naïve and memory CD8+ T cells. P = 0.0008 for regulatory CD4+ T cells; P = 0.04 for total CD8+ T cells; P = 0.0003 for memory CD8+ T cells. Numbers within bars in (c, e) denote the absolute number, ×10-7 and ×10-6, respectively, of each cell type.
Marques et al. Journal of Biology 2009 8:93 doi:10.1186/jbiol194