Dominant inhibition of Wnt-dependent transcription by Dsh mutants. Eight-cell embryos were injected (a) in one animal ventral blastomere or (b) in one vegetal ventral blastomere with -833pSia-Luc DNA (20 pg), mRNAs encoding Xwnt3a (5 pg) or Xwnt8 (2 pg), and Dsh-GFP, DsNLSm, Ds3 or DsSNLS mRNA (0.5 ng) as indicated. Luciferase activity was measured as described in Figure 5b.
Itoh et al. Journal of Biology 2005 4:3 doi:10.1186/jbiol20