RNAi profiles identify known and novel genes with related morphogenetic functions. Table headings are as defined as in Figure 2. (a, b) Profile I: binucleate cells that identified genes required for cytokinesis, as detected either in (a) both cell types or (b) a single cell type. (c, d) Profile II: F-actin phenotypes observed in both cell types identified genes with potentially conserved roles in F-actin dynamics. (c) Increased or polarized (uneven) accumulation of F-actin identified genes with potential roles in F-actin capping, severing or depolymerization. (d) Reduced F-actin and altered cell shape identified genes with potential roles in F-actin polymerization. (e, f) Profile III: a common RNAi phenotype observed in Kc167 cells was a change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. (e) Cases with phenotypes also observed in S2R+ cells identified genes involved in F-actin and microtubule regulation. (f) Cases with phenotypes observed only in Kc167 cells identified components of receptor signaling pathways. (g-i) Profile IV: RNAi phenotypes resulting in round, detached S2R+ cells. (g) Phenotypes detected in both S2R+ and Kc167 cells identified genes with probable indirect effects on cell adhesion and spreading, including roles in the cell cycle and cell viability; (h) RNAi phenotypes specific for S2R+ cells identified genes that may distinguish the flat S2R+ cell morphology, including genes encoding cell-matrix adhesion components. (i) Genes identified by a related RNAi phenotype, resulting in retracted (unspread but flat) S2R+ cells.
Kiger et al. Journal of Biology 2003 2:27 doi:10.1186/1475-4924-2-27