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Combined single-molecule force and fluorescence measurements for biology

Mark I Wallace, Justin E Molloy and David R Trentham*

Author Affiliations

National Institute for Medical Research, London NW7 1AA, UK

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Journal of Biology 2003, 2:4  doi:10.1186/1475-4924-2-4

Published: 14 April 2003


Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution.